Cell Lines and Cell Cultures
Lonza Walkersville P3 Primary Cell 384-well Nucleofector Kit
The P3 Primary Cell 384-well Nucleofector Kit is one of our five kits suited for high-throughput transfection of primary cells when working with the 384-well HT Nucleofector System.Benefits:High transfection efficiencies primary cells - Cells maintain functionality post transfectionSeamless integration into automated liquid handling environmentsConditions are transferable between 4D -ucleofector System, 96-well Shuttle System and 384-well Nucleofector SystemApplications:Transfection of low cell numbers down to 2 x 10⁴ primary cellsHigh-throughput applications, e.g. screens of arrayed cDNA, RNAi or CRISPR librariesContent:90 mL P3 Primary Cell Nucleofector Solution, 20 mL Supplement 1, 50 µg pmaxGFP Vector (1 μg/μl in 10 mM Tris pH 8.0), 10 384-well Nucleocuvette Plate (20 μl)
ATCC Campylobacter; jejuni; subsp. jejuni
Campylobacter jejuni subsp. jejuni strain RM3193 was isolated in 1994 from human diarrheal stool. This strain has applications in enteric disease research.
ATCC Priestia megaterium; 6 x 0.3mL
ATCC 14581-MINI-PACK™ consists of 6 ready-to-use vials of ATCC 14581™ frozen in 200 μL of glycerol stock, eliminating the need to rehydrate and culture the strain prior to use. Each vial is provided with a 2-D barcode for easy storage and tracking, as well as peel-off labels for fast and reliable recordkeeping.
ATCC U937-DC-SIGN; Histiocytic Lymphoma; Human (Homo sapiens)
U937-DC-SIGN was developed by transducing lymphocyte U-937 cells with the gene for human DC SIGN protein (Dendritic cell-specific intercellular adhesion molecule 3-grabbing non-integrin), a known attachment factor for Dengue viruses. The cloning vector used was pcDNA3 which contains SV-40 and CMV promoter regions. The U cell line that was established in 2008 from the stable transfection of U-937 cells (ATCC No. CRL-1593.2 isolated from a pleural effusion of a 37-year-old, white male patient with histiocytic lymphoma. This cell line was deposited by A. Desilva.
ATCC Hep 3B2.1-7; Hepatocellular Carcinoma; Human (Homo sapiens)
Hep 3B2.1-7 [Hep 3B, Hep-3B, Hep3B] is a cell line exhibiting epithelial morphology that was isolated from liver tissue derived from an 8-year-old, Black youth with liver cancer. This cell line contains an integrated hepatitis B virus genome.
Eurofins Discoverx cAMP Hunter CHO-K1 OXER1 Gi Cell Line, 2 vials. (95-0089C2)
The cAMP Hunter OXER1 Gi cell line is a stable, engineered cell line for use in studying drug candidates (small molecules or biologics) targeting GPCRs. This cell line enables assessment of ligand (e.g. 5-oxo-ETE) based activation of OXER1 GPCR activity via detection of decreasing levels of intracellular cAMP. This product uses EFC technology. The cells overexpress wild type OXER1, a naturally Gi/Go coupled GPCR. Upon activation of OXER1, the decrease in levels of intracellular cAMP leads to a decreased signal from EFC when the cAMP detection kit is applied. This stable modified cell line is provided as two vials of cryopreserved cells. For detailed product information, User Manual and datasheet, visit www.discoverx.com/gpcr.
Lonza Walkersville D-Hu Subcut Preadipo Diabet II
Poietics Diseased Human Preadipocytes (D-HprAD) are isolated from subcutaneous or visceral fat from diabetic Type I and diabetic Type II donors Preadipocytes from normal (non-diabetic) donors are available as a separate product Pertinent additional donor information including Body Mass Index (BMI) insulin dependence and age of diagnosis may be available by contacting Scientific Support Cryopreserved Poietics Diseased Human Preadipocytes (D-HprAD) are guaranteed to contain 1 million cells per ampoule and test positive for adipogenic differentiation All cells test negative for mycoplasma bacteria yeast and fungi HIV-1 hepatitis B and hepatitis C are not detected for all donors and/or cell lots A Certificate of Analysis is provided for each cell lot purchased
Amsbio LLC JURKAT FFPE Cell Pellet Slide (5-pack)
FFPE cell line sections are manufactured using a proprietary technology that maintains both cell morphology and preserves nucleic acids and proteins. Available from over 120 tumor cell lines, these FFPE sections make for affordable and consistent controls without compromising quality or consistency. Using cell line controls overcomes issues experienced with traditional cell or tissue-based control specimens such as intra specimen variation, lot-to-lot variations and inconsistent biomolecule quality. Also available in scroll & block formats - please enquire.
ATCC Staphylococcus epidermidis; Quantitative DNA
Quantitative genomic DNA from Staphylococcus aureus FDA strain PCI 1200 (ATCC 12228) can be used for assay development, verification, validation, and monitoring of day-to-day test variation and lot-to-lot performance of molecular-based assays. The quantitative format allows for the generation of a standard curve for quantitative PCR (qPCR) to determine bacterial load and for other molecular biology applications.
Eurofins Discoverx ChemiBrite EP3 Prostanoid Receptor Stable Cell Line, 2 vials. (HTS092L)
The ChemiBrite EP3 Prostanoid Receptor cell line is a stable, engineered cell line for use in studying drug candidates (small molecules or biologics) targeting GPCRs. This cell line enables assessment of ligand (e.g. prostaglandin E2) based activation of PTGER3 GPCR activity via detection of increasing levels of intracellular calcium. The included cell line overexpresses PTGER3 alongside exogenous high levels of a promiscuous G-protein, coupling the receptor to the calcium signaling pathway. ChemiBrite cells also express a clytin variant, allowing for luminescent as well as fluorescent signal detection. Upon activation of PTGER3, the resulting calcium activity can be assayed in either detection mode. This stable modified cell line is provided as two vials of cryopreserved cells. For detailed product information, visit www.discoverx.com/targets/gpcr-target-biology.
Eurofins Discoverx PathHunter RXFP3 Total Internalization U2OS Cell Line, 2 vials. (93-0948C3)
The PathHunter RXFP3 Total Internalization cell line is a stable, engineered cell line for use in studying drug candidates (small molecules or biologics) affecting GPCR activation and internalization. This cell line uses EFC technology to measure ligand-induced internalization of RXFP3 via detection of localization to the early endosome. The included cell line overexpresses ProLink-tagged RXFP3 and an EA-tagged early endosome protein. Upon ligand-induced receptor activation, resulting internalization of the RXFP3 receptor to the early endosome leads to production of EFC signal. This stable modified cell line is provided as two vials of cryopreserved cells. For detailed product information, User Manual and datasheet, visit www.discoverx.com/gpcr.
ATCC Staphylococcus aureus; subsp. aureus
Staphylococcus aureus subsp. aureus strain HDE288 is a methicillin-resistant bacterium that was isolated from the pneumology ward of a hospital in Lisbon, Portugal. This strain has applications in antimicrobial resistance research, drug development, and infectious disease research.
ATCC MCF 10A Ecadherin; EmGFP Reporter; Breast Human
For-profit organizations: For every order of this item, you must provide a signed Material Transfer Agreement Addendum for Screening Application. We cannot ship this item until we receive this addendum. The person signing the addendum as the principal investigator must match the end user as listed on the applicable sales order for the item.Email the signed addendum to SalesPermits@atcc.org with a reference to both your account and sales order numbers. Once received, your addendum will be reviewed, and this item will be released for shipment if all requirements are met. Additional fees may apply if this product is being used for a screening use (ATCC ACS-2103F), and these fees will be applied after your order is confirmed. If you need assistance with your order, please contact our Customer Care team.
ATCC Giardia intestinalis; Portland-1
Giardia intestinalis strain Portland-1 was isolated in Portland, Oregon. This parasitic protozoan has applications in food and waterborne pathogen research.